Analysis Note

The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.

Application

Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.

Features and Benefits

Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation

Principle

The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.